Journal: Drug Delivery and Translational Research

Article Title: Subcellular detection of PEBCA particles in macrophages: combining darkfield microscopy, confocal Raman microscopy, and ToF–SIMS analysis

doi: 10.1007/s13346-022-01128-3

Figure Lengend Snippet: Enhanced darkfield microscopy and hyperspectral microscopy of NR8383 alveolar macrophages loaded with different PEBCA particles. Cells were loaded with 100 µg/mL of the indicated particles for 4 h. ( A – C ) Cells loaded with PEBCA NR668 IR. ( A ) DFM image, ( B ) fluorescence image, ( C ) overlay of ( A ) and ( B ). Note that light-scattering regions not always correspond to fluorescent areas. ( D – G ) Analysis of cells with DFM and hyperspectral imaging (HSI); two regions of interest (ROI) were analyzed in each picture. ( D ) Untreated control cells, ( E ) cells loaded with PEBCA NR668 IR, ( F ) cells loaded with PEBCA CBZ, ( G ) cells loaded with PEBCA. HSI curves from all regions 1 and 2 were superimposed. Despite some spectral disparity between 450 and 550 nm, we were unable to differentiate between differently treated cells ( H )

Article Snippet: DFM plus HSI plus Raman images were taken with an Olympus BX43 microscope equipped with an enhanced darkfield condenser, a hyperspectral imaging device, and ENVI 4.8 software (CytoViva Inc., Auburn, AL, USA), and an integrated confocal Raman unit operated with LabSpec 6 software (Horiba Xplora Plus, HORIBA Jobin Yvon GmbH, Bensheim Germany).

Techniques: Microscopy, Fluorescence, Imaging